COMPARATIVE STUDIES OF KINETIC PARAMETER OF SOME DETOXIFICATION ENZYMES ISOLATED FROM HIND GUT OF Zonocerus variegatus Linnaeus, 1758
Keywords:
Grasshopper, Catalase, Glutathione-S-transferase, Lysozyme, Polyphagous, PeroxidaseAbstract
AIMS: Grasshopper (Zonocerus variegatus) is a polyphagous insect that feeds on wild and cultivated and uncultivated crops thus causing extensive damage in their feeding process. Understanding the presence and expressions of various detoxification enzymes (chemical defense) in the gut of Zonocerus variegatus (Z.v) may help in understanding how they cope with allelochemicals encountered in their feeding process.
METHODOLOGY: The grasshoppers were collected and dissected to obtain the hindgut. The hindgut was homogenized n Tris-HCl buffer (pH 9.6) and centrifuged at 10,000 g for one hour. The supernatant was collected and stored as crude enzyme. Assay for the presence and quantification of the following enzymes Glutathione-S-transferase (GST), Peroxidase, Lysozyme, Catalase and Superoxide Dimutase (SOD) were carried out. Specific activity and kinetic parameters of the enzymes were also determined.
RESULTS: GST expression is the lowest amongst the antioxidant enzyme assayed for with specific activity of 2.32 ± 0.6 Units/mg protein, while catalase was the most expressed with specific activity of 3827.37 ± 201.11 Units/mg protein. The order of expression form largest to lowest were catalase > superoxide dismutase> lysozyme> peroxidise> GST.
CONCLUSION: This study was able to establish that Zonocerus variegatus contain varying quantity of detoxification enzymes, which helps the insect to eliminate both exogenous and endogenous toxins.
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References
Adeleke, G. E., Adedosu, O. T., Olayioye, A., Olaniyi, A. A., Aderoju, V. B., and Akintaro, O. O. (2021). In-vitro Pesticidal effects of Water hyacinth leaf and Cashew nut shell extracts against Acanthoscelides obtectus and Zonocerus variegatus. IOSR Journal of Environmental Science, Toxicology and Food Technology (IOSR-JESTFT), 15(5): 36-48.
Adeyi, A. O., Akozi, G. O., Adeleke, M. A., Agbaogun, B. K. O., and Idowu, A. B. (2015). Induction and activity of glutathione S-transferases extracted from Zonocerus variegatus (Orthoptera: Pyrgomorphidae) exposed to insecticides. International Journal of Tropical Insect Science, 35(1): 27-33
Adewale, I. O., and Afolayan, A. (2006). Studies on glutathione transferase from grasshopper (Zonocerus variegatus). Pesticide Biochemistry and Physiology, 85(1): 52-59.
Agunbiade, J. O., Adewale, I. O., and Afolayan, A. (2014). Kinetic analysis of glutathione transferase from rats exposed to sub-lethal and lethal concentrations of lead acetate. Ife Journal of Science, 16(3), 417-423.
Ames, B.N., Prolet, M., and Gold, I.S. (1990). Nature’s chemicals and synthetic chemicals: Comparative toxicology. Proc. Natl. Acad. Sci. USA. 87: 7782-7786
Augustyniak, M., Babczynska, A., and Augustyniak, M. (2009). Does the grasshopper Chorthippus brunneus adapt to metal polluted habitats? A study of glutathione‐dependent enzymes in grasshopper nymphs. Insect Science, 16(1), 33-42.
Bessie, I. U., and Agboola, F. K. (2013). Detoxification of cyanide in insects. I. Purification and some properties of rhodanese from the gut of the variegated grasshopper Zonocerus variegatus (Orthoptera: Pyrgomorphidae). International Journal of Tropical Insect Science, 33(3), 153-162.
Bradford M.M (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-54.
Chance, B. and Maehly, A.C. (1955) Assay of Catalase and Peroxidase. Methods in Enzymology, 2, 764-775. http://dx.doi.org/10.1016/S0076-6879(55)02300-8
Edwin, H.L and Derek, T.A.L (1974). An accounting of Horseradish Peroxidase Isoenzymes Associated with the Cell Wall and Evidence that Peroxidase Does Not Contain Hydroxyproline. Plant Physiology. 54: 870-876
Engel, P., and Moran, N. A. (2013). The gut microbiota of insects–diversity in structure and function. FEMS microbiology reviews, 37(5), 699-735.
Epel, D. (1998). Use of multidrug transporters as first lines of defense against toxins in acquatic organisms. Comp. Biochem. & Physiol. Part A. 12:23-28
Famutimi, O. G., and Adewale, I. O. (2021). Induction and catalytic properties of grasshopper (Zonocerus variegatus) glutathione transferase fed on different food plants. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 241: 108970.
Francis, F.,Haubruge, E.,Gaspar,C., and Dierickx, P.J.,(2001).Glutathione S-transferases of Aulacorthumsolani and Acyrthosiphonpisum: partial purification and characterization, Comp. Biochem. Physiol. B 129, 165-171
Gonzalez, F.J., and Nebert, D.W., (1990). Evolution of the P450 gene super family: Animal and plant ‘warefare’, molecular driven and human genetic differences in drug oxidation. Trends in Genetics. 6:182-186
Guengerich, F.P., (1990). Enzymatic oxidation of xenobiotics chemicals.CRCCrit. Rev. Biochem. Mol. Biol. 25:97-153
Habig, W.H., Pabst, M. J., and Jakoby, W.B. (1974).Glutathione S-transferases: the first enzymatic step in mercapturic acid formation. J. Biol. Chem. 249, 7130-7139
Hayes, J.D., and Mclellan, L.I., (1999). Glutathione and glutathione dependent enzymes represent a co-ordinately regulated defense against oxidative stress, Free radic. Res. 31: 273-300
Hayes, J.D., and Pulford, D. J., (1995). The glutathione S-transferases supergene family: Regulation of GSH and the contributions of the isoenzymes in cancer chemoprotection and drug resistance. CRC Crit. Rev. Biochem. Mol. Biol. 30:445-600
Higgins, L. G., and Hayes, J. D. (2011). Mechanisms of induction of cytosolic and microsomal glutathione transferase (GST) genes by xenobiotics and pro-inflammatory agents. Drug metabolism reviews, 43(2), 92-137.
Kennedy, C. J., and Tierney, K. B. (2012). Xenobiotic protection/resistance mechanisms in organisms. In Environmental Toxicology: Selected Entries from the Encyclopedia of Sustainability Science and Technology (pp. 689-721). New York, NY: Springer New York
Li,W., Schuler, M.A., and Berenbaum, M.A. (2003). Diversification of furanocoumaris metabolizing CytP450 monooxygenases in 2 papilionids: Specificity and substrate encounter rate. PNAS 100: 14593-1459
Litman, T., Druley, T.E., Stein, W.D, and Bates, S.E., (2001). From MDA to MXR: new understanding of multidrug resistance systems, their properties and clinical significance. Cellular & Mol. Life Sci. 58: 931-959
Marinka, G., Gilbert, O.F, and Andreas.P, (2001).A Catalase-peroxide from a newly isolated thermoalkaliphilic bacillus sp. with potential for the treatment of textile bleaching effluents. Extremophiles. 5: 423-429
Piwowarska, D., and Kiedrzyńska, E. (2022). Xenobiotics as a contemporary threat to surface waters. Ecohydrology & Hydrobiology. 22(2): 337-354.
Prapanthadara, L., Promtete, N., Koottathep, S., Somboon, P., and Ketterman, A.J. (2000). Isoenzymes of glutathione S- transferase from the mosquito Anopheles dirus species B: the purification, partial characterization and interaction with various insecticides. Insect Biochem. Mol. Biol. 30, 395
Prestamo,C. and Manzano. P. (1993). Peroxidase of Selected Fruits and Vegetables and the possible use of Ascorbic acid as an Antioxidant. HortScience .28(1): 48- 50
Saha, D. (2016). Biochemical insecticide resistance in tea pests. Insecticides Resistance, 347
Sheehan, D., Meade, G., Foley, V.M., and Dowd, C.A., (2001). Structure, function and evolution of glutathione transferases: implications for classification of non-mammalian members of an ancient enzyme super family. Biochem. J. 360: 1-16
Sherratt, P. J., and Hayes, J. D. (2001). Glutathione S‐transferases. Enzyme systems that metabolise drugs and other xenobiotics, Ch. 9: 319-352.https://doi.org/10.1002/0470846305.ch9
Shugar. D. (1952). The measurement of lysozyme activity and the ultra- violet inactivation of lysozyme. Biochem. Biophys. Acta 8:302-309.
Ughachukwu, P. O., and Unekwe, P. C. (2012). Efflux Pump. Mediated Resistance in Chemotherapy. Annals of Medical and Health Sciences Research. 2(2): 191-198.
van Huis, A. (2022). Cultural significance of locusts, grasshoppers, and crickets in sub-Saharan Africa. J Ethnobiology Ethnomedicine 18:24 https://doi.org/10.1186/s13002-022-00524-wM
Wang, Y., Huang, X., Chang, B. H., and Zhang, Z. (2020). The survival, growth, and detoxifying enzyme activities of grasshoppers Oedaleus asiaticus (Orthoptera: Acrididae) exposed to toxic rutin. Applied Entomology and Zoology. 55: 385-393
Zhang, Y., Sun, G., Yang, M., Wu, H., Zhang, J., Song, S., … Guo, Y. (2011). Chronic accumulation of cadmium and its effects on antioxidant enzymes and malondialdehyde in Oxya chinensis (Orthoptera: Acridoidea). Ecotoxicology and Environmental Safety, 74(5), 1355–1362.