AMPLIFICATION AND CLONING OF SF3B3 GENE INTO PMXS-FLAG VECTOR USING RECOMBINATION CLONING KIT

Authors

  • Haibing Xiao Department of Microbial and Biochemical Pharmacy, College of Pharmacy, Jinan University, Guangzhou, China
  • Jin Zeng Department of Microbial and Biochemical Pharmacy, College of Pharmacy, Jinan University, Guangzhou, China
  • Gan Yu Department of Microbial and Biochemical Pharmacy, College of Pharmacy, Jinan University, Guangzhou, China

Keywords:

SF3B3, alternative splicing, homologous recombination, colorectal cancer, hepatocellular carcinoma

Abstract

Alternative splicing is a crucial process for regulating gene expression in eukaryotes, and dysregulation of this process has been implicated in multiple diseases, including cancer. SF3B3 is a key splicing factor that has been shown to be upregulated in various cancers, including colon cancer, renal cell carcinoma, hepatocellular carcinoma, and breast cancer. Mutations in SF3B3 have been found to promote the proliferation, colony formation, migration, and invasion of liver cancer cells. In this study, we report the successful construction of an overexpression vector for SF3B3 using homologous recombination. The pMXsSF3B3 recombinant plasmid serves as a foundation for further investigation into the function of SF3B3 in tumorigenesis. The link between splicing and transcription and the potential of spliceosome components as therapeutic targets for cancer treatment is highlighted by this study.

Published

2023-08-02

How to Cite

Haibing , X., Jin , Z., & Gan , Y. (2023). AMPLIFICATION AND CLONING OF SF3B3 GENE INTO PMXS-FLAG VECTOR USING RECOMBINATION CLONING KIT . Interdisciplinary Journal of Agriculture and Environmental Sciences (IJAES), 10(1), 11–14. Retrieved from https://sadijournals.org/index.php/IJAES/article/view/9

Issue

Vol. 10 No. 1 (2023)

Section

Original Peer Reviewed Articles
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